Description
TheApplied Biosystems 7500 Fast Dx Real-Time PCR Instrument with SDS Software is a real-time nucleic acid amplification and five-color fluorescence detection system available for in vitro diagnostic use.
The 7500 Fast Dx Real-Time PCR Instrument delivers the performance required for high-quality results in a 96-well format.
• 96-well format eases plate setup if automation is not available
• Accommodates tube strips, which can be capped immediately after pipetting each sample
• Fast mode completes runs in less than forty minutes
• Optional standard mode facilitates use of standard length real-time PCR assays without changing thermal cycling parameters
• 5-color variable excitation enables multiplex assays
Convenient 96-well Format
The 7500 Fast Dx Real-Time PCR Instrument minimizes the effort required to perform sample setup while also allowing the use of tube strips.
Intuitive Software to Run Pre-optimized Protocols
The Applied Biosystems 7500 Fast Dx Real-Time PCR Instrument Sequence Detection Software Security, Auditing, and E-Signature module offers the flexibility to develop assays and also run pre-optimized protocols for users operating in a secure environment.
Instrument Service and Support
The 7500 Fast Dx Real-Time PCR Instrument is supported by our AB Assurance Service Plan for Diagnostics, which includes:
• Instrument Installation Qualification, Operation Qualification, and Performance Qualification (IQ⁄OQ⁄PQ)
• Spectral calibration and software setup
• Emergency repair, including parts and labor, and OQ⁄PQ after any major part change
• Planned maintenance every 12 months
Europe: For in vitro Diagnostic Use. The Applied Biosystems 7500 Fast Dx Real-Time PCR Instrument meets the requirements of the in vitro Diagnostic Medical Devices Directive (98⁄79⁄EC). The CE IVD-registered 7500 Fast Dx Real-Time PCR Instrument is for distribution and use in specific European countries only.
USA: For in vitro Diagnostic Use. The 7500 Fast Dx Real-Time PCR instrument is 510(k)-cleared (K082562). The customer is responsible for any validation of assays and compliance with any regulatory requirements that pertain to their procedures and instrument use.
The Applied Biosystems 7500 Fast Dx Real-Time PCR Instrument is an affordable solution for budget-conscious labs. The four color detection system gives users the flexibility to run a variety of applications including SNP genotyping, gene expression, plus/minus assays, pathogen quantitation and more. The instrument is equipped with a CCD camera for precision optics and comes with advanced multi-componenting algorithms for accurate and reproducible results.
The ABI 7500 Real-Time PCR system is an affordable solution for budget-conscious labs. The four color detection system gives users the flexibility to run a variety of applications including SNP genotyping, gene expression, plus/minus assays, pathogen quantitation and more. The instrument is equipped with a CCD camera for precision optics and comes with advanced multi-componenting algorithms for accurate and reproducible results.
Specifications Applied Biosystems 7500 Fast Dx Real-Time PCR Instrument
| Calibrated Dye: | TAMRA, Cy3, FAM, SYBR Green, Texas Red, JOE, ROX, Cy5, NED, VIC |
|---|---|
| Capacity: | 12 x 8-tube strips, 1 x 96-well plate (Fast) |
| Dimensions: | 34 cm (13.39 in) (W) x 49 cm (19.29 in) (H) x 45 cm (17.72 in) (D) |
| External Computer: | Laptop computer provided |
| Format: | 96-well plate, 8-well strips |
| Optics: | CCD Camera, Halogen light source, Five excitation and five emission filters |
| Passive Reference Dye: | ROX (Separate Tube), Other Passive Reference Dye, ROX (Pre-mixed) |
| Peak Block Ramp Rate: | 5.5°C⁄sec |
| Precision: | Distinguish between 5,000–10,000 genome equivalents (two-fold copy number difference) with 99.7% confidence |
| Reaction Speed: | Fast, Standard |
| Reaction Volume Range: | 10-30 µl |
| Run Time: | 36 min (Fast), <2 hrs (Standard) |
| Sample Ramp Rate: | Fast mode: ± 3.5°C⁄sec, Standard mode: ± 1.6°C⁄sec |
| Temperature Accuracy: | ±0.5°C |
| Temperature Range (Metric): | 4.0-99.9 °C |
| Temperature Uniformity: | ±1°C |
| Thermal Cycling System: | Peltier-Based System |
| Throughput: | 4 to 5 96-well plates per day |
| Weight: | 34 kg (75 lb) |
| For Use With (Equipment): | 7500 Fast Dx System |
| Dynamic Range: | 9 logs of linear dynamic range |
| Includes: | Laptop Computer |
| Sensitivity: | Down to 1 copy of human RNAse P gene |
Includes
– (1) ABI Biosystems 7300 Real-Time PCR System main unit
– (1) Laptop computer with SDS 1.4 software
– USB and power cable